Fig. 4

Eight differentially expressed genes are potentially involved in LMNA-related cardiomyopathy. a Differentially expressed genes that passed the filtering criteria were input into Ingenuity Pathway Analysis (IPA). Of these significantly differentially expressed genes for each group, there were eight genes in the intersection (arrow) between B and C, but not A, where A = Unrelated vs. Controls, B = Unrelated vs. Patients, C = Controls vs. Patients; b Gene symbols, names, and RNA-seq values for eight genes found in the intersection between groups B and C, but not A; *Expression in patient: -: decreased, +: increased. c RNA-seq validation of Insulin Growth Factor binding protein 5 (IGFBP5) transcript levels by quantitative PCR (qPCR). qPCR was performed on cDNA generated from unrelated, control and patient fibroblasts to measure IGFBP5 transcript level. There was no statistically significant difference in IGFBP5 transcript levels (average QGE +/− SEM) between all groups [F (2,6)= 0.37, p = 0.71]. Statistical analysis was performed using One-way ANOVA followed by Tukey post hoc test