Table 1 The PCR primers and conditions for HSF4 primers
From: Novel mutations in HSF4 cause congenital cataracts in Chinese families
Exon | Primer name | Primer sequence(5′-3′) | Amplicon size (bp) | PCR condition |
|---|---|---|---|---|
4, 5 | HSF4e4/5F | GGACCCAAGAGTGAGCATGA | 481 | 58 °C/GC Buffer1 |
HSF4e4/5R | CCCTCCTCCTCTTTGCTCAT |