Fig. 3

Transversely cut skeletal muscle tissue shows dystrophic features on HE staining in both DMD and β- sarcoglycanopathy (Fig I & Q) as against normal muscle tissue (Fig A). Immunohistochemically, antibodies against dystrophin (dys1,2,3) and sarcoglycans (α,β,γ,δ) shows preserved expression along the membrane in all the fibres (Fig B-H) in normal muscle tissue, while total loss of expression for dystrophin (Fig J,K,L) and preserved expression for sarcoglycans (Fig M,N,O,P) indicates the diagnosis of the DMD. Note: Preserved expression of dystrophin (Fig R,S,T) and δ &γ sarcoglycans (Fig W,X) reduced α-sarcoglycan (Fig U)and complete absence of β-sarcoglycan (Fig V) in a case of b-sarcoglycanopathy