Fig. 5

Binding of nuclear proteins from K562 cells with SNPs rs2855126, rs11036496, and rs4348933 determined by EMSA. Lanes 1, 4, and 7: in the absence of nuclear extract, biotin-labeled probe shows no retardation; lanes 2, 5, and 8: in the presence of nuclear extract, biotin-labeled probe shows retardation and gel shift due to the slower migration of protein-probe complex; and lanes 3, 6, and 9: in the presence of nuclear extract and unlabeled probe (as competitor), biotin-labeled probe shows no retardation. For both probe and nuclear extract, + indicates presence and - indicates absence.