Table 1 Real-Time PCR: nuclear and mtDNA native and WGA samples
From: Multiple strand displacement amplification of mitochondrial DNA from clinical samples
| Â | Native input nuclear DNA (ng) | Nuclear DNA in 50 uL WGA (ng) | Fold nuclear DNA amplification by WGA | mtDNA input nuclear DNA (ng) | mtDNA in 50 uL WGA (ng) | Fold mtDNA amplification by WGA | Â |
|---|---|---|---|---|---|---|---|
CB 4 | 6.80E-01 | 2.80E+04 | 4.12E+04 | 1.28E+00 | 1.38E+04 | 1.08E+04 | CB 4 |
CB 1 | 1.65E+00 | 1.98E+04 | 1.20E+04 | 2.38E+00 | 3.66E+04 | 1.54E+04 | CB 1 |
CS 3 | 2.03E+00 | 1.07E+04 | 5.25E+03 | 3.50E+00 | 2.82E+03 | 8.05E+02 | CS 6 |
CS 6 | 2.05E+00 | 8.38E+03 | 4.08E+03 | 7.65E+00 | 2.94E+03 | 3.84E+02 | CS 8 |
CS 2 | 4.28E+00 | 1.11E+04 | 2.58E+03 | 8.12E+00 | 1.50E+04 | 1.85E+03 | CS 1 |
CS 1 | 5.14E+00 | 3.47E+03 | 6.75E+02 | 8.13E+00 | 1.93E+03 | 2.38E+02 | CS 3 |
CS 8 | 7.29E+00 | 6.15E+03 | 8.44E+02 | 1.29E+01 | 5.47E+03 | 4.24E+02 | CS 2 |
CB 2 | 1.23E+01 | 6.37E+03 | 5.19E+02 | 1.26E+02 | 1.17E+05 | 9.24E+02 | CS 5 |
CB 3 | 6.52E+01 | 7.82E+03 | 1.20E+02 | 2.15E+02 | 3.07E+04 | 1.43E+02 | CB 2 |
CS 5 | 9.37E+01 | 2.83E+04 | 3.02E+02 | 2.92E+02 | 3.87E+04 | 1.33E+02 | CB 3 |
CS 7 | n/a | 5.82E+03 | n/a | n/a | 3.06E+03 | n/a | CS 7 |
PB 2 | 1.46E-01 | 9.06E+03 | 6.19E+04 | 0.04 | 8.33E+03 | 2.10E+05 | PB 4 |
PB 4 | 1.56E-01 | 7.43E+03 | 4.75E+04 | 0.05 | 2.84E+03 | 6.08E+04 | PB 2 |
PT 3 | 1.70E-01 | 8.82E+02 | 5.19E+03 | 0.26 | 1.26E+03 | 4.91E+03 | PB 1 |
PT 2 | 2.26E-01 | 5.47E+03 | 2.42E+04 | 0.27 | 4.32E+03 | 1.60E+04 | PB 3 |
PB 3 | 2.36E-01 | 2.95E+03 | 1.25E+04 | 0.41 | 8.03E+03 | 1.94E+04 | PT 2 |
PB 1 | 4.77E-01 | 2.85E+03 | 5.97E+03 | 0.77 | 3.51E+03 | 4.58E+03 | PT 3 |
PT 1 | 5.33E-01 | 4.41E+03 | 8.27E+03 | 1.45 | 4.08E+03 | 2.81E+03 | PT 1 |
PB 5 | n/a | 4.59E+03 | n/a | n/a | 1.60E+04 | n/a | PB 5 |