Figure 3

Analysis of FGF10 transcript applying long range RT-PCR. In order to analyze for transcripts that may include the entire intron 2, a long range RT-PCR protocol was established that works to amplify the 6.2 kb-fragment spanning from exon 2 to exon 3 using gDNA as template. Applying the optimized conditions to amplify the FGF10 transcript, no accordant PCR product was present. 1, 3: patient; 2, 4: control; MW: molecular weight marker.