GATA4 exons | Exon size (bp) | Primer | Primer length | Primer sequence (5'-3') | Melting temperature | PCR product length (bp) |
---|---|---|---|---|---|---|
exon 1 | 61 | GT4x1F | 20 | cttgcacgtgactcccacag | 65°C | 250 |
 |  | GT4x1R | 20 | aagcaaaggcggagaagctc |  |  |
exon 2 | 1073 | GT4x2-1F | 21 | tctctttctgtcgttcctctt | 65°C | 600 |
 |  | GT4x2-1R | 20 | gcacgtagactggcgaggac |  |  |
 |  | GT4x2-2F | 21 | ggaccatgtatcagagcttgg | 65°C | 641 |
 |  | GT4x2-2R | 20 | gccctcgcgctcctactcac |  |  |
exon 3 | 167 | GT4x3F | 24 | agtcagagtgaggaagagcaagag | 65°C | 541 |
 |  | GT4x3R | 21 | cagtttctgtgtgccgaagag |  |  |
exon 4 | 126 | GT4x4F | 20 | ccagccctgcctcccgttag | 65°C | 294 |
 |  | GT4x4R | 23 | gaggactgagagatgggcatcag |  |  |
exon 5 | 88 | GT4x5F | 22 | agtagccatcacatcacacagg | 65°C | 504 |
 |  | GT4x5R | 19 | aaagctcccaacacgttcc |  |  |
exon 6 | 149 | GT4x6F | 20 | gtttgtccctgccgctgatt | 65°C | 247 |
 |  | GT4x6R | 20 | gcagtcggcctccccacaaa |  |  |
exon 7 | 1708 | GTx7-1F | 19 | acaaggctatgcgtctccc | 60°C | 289 |
 |  | GTx7-1R | 20 | ctgagaaaatccaacacccg |  |  |
 |  | GT4x7-2F | 20 | gcgtaatcttccctcttccc | 65°C | 291 |
 |  | GT4x7-2R | 19 | gggacaaggacatcttggg |  |  |
 |  | GTx7-3F | 20 | gtcgacaatctggttagggg | 60°C | 281 |
 |  | GTx7-3R | 20 | gtacatggcaaacagatgcc |  |  |
 |  | GTx7-4F | 20 | gaggatctgagaacaagcgg | 60°C | 270 |
 |  | GTx7-4R | 20 | cagctgcattttgatgaggc |  |  |
 |  | GTx7-5F | 22 | aaattgtggggtgtgacataca | 60 °C | 577 |
 |  | GTx7-5R | 22 | gttgcagaatctctggcttttt |  |  |
 |  | GTx7-6F | 22 | ctgtctgtctgctcctcctagc | 65°C | 586 |
 |  | GTx7-6R | 22 | acctcccagtgaagaccactaa |  |  |