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Table 1 Primers and DGGE screening conditions

From: Three novel and the common Arg677Ter RP1 protein truncating mutations causing autosomal dominant retinitis pigmentosa in a Spanish population

Primers*

cDNA Sequence

Fragment size (bp)

Gradient %**

Running T (°C)

Forward 5'-GTAATAACTCTGGAACTGACAA-3'

1998–2308

350

40–70

50

Reverse 5'-GC-GGACTATCTGAACTTTGCACTA-3'

    

Forward 5'-CAGGTATCAAGATGGACAGC-3'

2197–2461

304

40–70

50

Reverse 5'-GC-TGAACCTTGGAATTTTGAGTAG-3'

    
  1. * (GC) = 5'-CGCCCGCCGCGCCCCGCGCCCGGCCCGCCGCCCCCGCCCG-3' ** 100% denaturant = 7M urea and 40% (v/v) formamide in TAE buffer
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BMC Medical Genetics

ISSN: 1471-2350

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