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Table 2 SNRPN and snoRNA expression analysis with quantitative RT-PCR

From: Molecular breakpoint cloning and gene expression studies of a novel translocation t(4;15)(q27;q11.2) associated with Prader-Willi syndrome

Amplification product PWS Normal control t-PWS (4;15) PWS triplication t-PWS intron 2 t-PWS (4;15)
  LCL LCL LCL LCL FB FB
SNURF Ex 2 0.0001 0.53 0.54 2 0.002 0.56
SNURF Ex 3 0.0002 1.1 1.43 6.4 0.0004 0.56
HBII-437 0.00003 0.86 1.13 4.9 0.00008 0.1
SNRPN 14/15 0.0013 4.7 4.91 17.23 - -
SNRPN 19/20a 0.005 1.34 0.003 4.7 0.007 0.007
HBII-438 0.03 1.5 0.02 6.2 0.07 0.07
PWCR1/HBII-85 0.03 3.7 0.02 16.8 0.04 0.06
  1. Sample identification: PWS: PWS with an IC microdeletion (patient E in [5]); t(4;15) PWS: the PWS case reported here; PWS-triplication: intrachromosomal triplication of the PWS region [33]; t-PWS intron 2: previously reported PWS case with t(4;15)(q27;q11.2) and breakpoint in SNRPN intron 2 [26, 27]; LCL, lymphoblastoid cell line, FB, fibroblast strain. The numbers represent the ratio of target product to GAPDH control product.