Figure 8

Transmission EM of CFBE41o^- cells transfected at the (6+/1-) lipid/DNA ratio and fixed 96 h after the start of transfection. (A)-(C) Large vesicles filled with labelled complexes in the cytoplasm of a transfected cell; (C) a higher magnification of (B). Arrows indicate a fusion site between a large vesicle and a lysosome. (D)-(F) labelled membranous body and lysosome in the cytoplasm of a transfected cell; (F) a higher magnification of a membranous body, in which the lipid/DNA complexes appeared partially degraded. ly: lysosome; mb: membranous body; mbv: membrane-bound vesicles. Bars: (A)-(E): 300 nm; (F): 100 nm.