Table 2 Genotyping procedure for the studied SNPs
Gene | SNP ID | Primer sequence (5′-3′) | Genotyping procedure |
|---|---|---|---|
SIM2 | rs2269188 | F: CTCACCACGAGCTACCTGAA | RFLP analysis of PCR product using Bsu36I |
R: GACCAGGAGAGGGTTTGGTC | |||
rs2070650 | F: CAGTGCCATGGCCTTTTTAGA | DNA sequence analysis in ABI prism 3130 Genetic Analyzer using Big Dye sequencing kit v3.1 followed by analysis using Sequencing Analysis software v 5.2. Electropherograms obtained were further analyzed by Mutation Surveyor Demo V3.24 software to check for new mutation | |
rs79727992 | R: CCAATACACACACAGCACCC | ||
rs16994404 | |||
rs77335240 | |||
rs78455239 | |||
rs79022672 | |||
ETS2 | rs34373350 | F: GGGGGTTTCCTTCCAGACT | |
rs11700777 | R: CTGATTGGGAAAGTCACGTGGG | ||
rs114481523 | |||
rs73450556 | |||
rs77688599 | |||
rs60277131 | |||
rs78391361 | |||
rs1803557 | |||
rs34472454 | |||
rs113798497 | |||
rs374575 | F: GTCTGATCAAGAGGCCCAAG | ||
rs2070529 | R: CAGAACCACTGGGGAATGAG | ||
rs2070530 | |||
rs2070531 | |||
rs434421 | |||
rs8128227 | |||
rs6517481 | |||
rs79863249 | |||
rs60538921 | |||
rs7276961 | |||
rs1051475 | F: TGTGTTTCTCCGACAGCTCA | ||
rs1051476 | R: TTTCATCAAGACCCCTACCG | ||
rs72094783 | |||
rs116542090 | |||
rs74551083 | |||
rs11540409 | |||
rs11254 | F: CCATTCATTCGGAGAAAACG | RFLP analysis of PCR product using TaqI | |
R: AAGGCCACGCAGCTAGTAAA | |||
rs711 | F: GCAACGGCACAGCTAATTCT | RFLP analysis of PCR product using MspI | |
R: AAATACAACTGTTAAGGGATTCTGA |