Figure 3From: Beta catenin and cytokine pathway dysregulation in patients with manifestations of the "PTEN hamartoma tumor syndrome" Cytokine disregulation on peripheral blood cells of PHTS patients a) Real Time PCR quantification analysis of IL10 and TNFα mRNA. b) Western blot assay of TNFR1 and TNFR2. H1-5, H6-10, H11-15, H15-20: mixes of healthy subjects; Hm: mean value between all healthy samples used as calibrator to measure the relative expression; PHTS1, PHTS2 and PHTS3: patients affected by PHTS syndrome, as reported in Table 1. c) RT-PCR analysis of the TNFRSF1Aβ isoform. S.M.: genomic molecular weight marker; B: amplified sample without cDNA template. d) sequence analysis of the amplified fragments showed in Figure 3d. The genomic sequence GenBank accession number is: EU927389. The underlined nucleotide are those showed in the electropherogram.Back to article page