Figure 3
From: PTPRF is disrupted in a patient with syndromic amastia
Expression studies of the PTPRF. (A) Relative quantification using 5'PTPRF probe (proximal to the breakpoint) showed that the expression level in the proband was significantly decreased compared with that in a control, the proband's mother and sister. (B) Relative quantification using the 3'PTPRF probe (distal to the breakpoint) showed significantly decreased expression in the proband. Data are presented as mean ± SEM. ** indicates P < 0.01 (independent 1-tailed t-test) using GAPDH as a control. (C) Western blot analysis showing levels of the PTPRF protein (150 kDa) and GAPDH (38 kDa). (D) Western blot analysis indicates nearly absent PTPRF in the proband. Data are represented as mean ± SEM. * indicates P < 0.05 (independent 1-taliled t-test) using GAPDH as a control.