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Table 1 Primer sets used in this study

From: Influence of MIF polymorphisms on CpG island hyper-methylation of CDKN2A in the patients with ulcerative colitis

Primer sets for MSP

 p14-UM_F

5′-gagtttggttttggaggtgg-3’

 p14-UM_R

5′-aaccacaacaacaaacacccct-3’

 p14-M_F

5′-tgagtttggttttggaggtgg-3’

 p14-M_R

5′-aaaaccacaacgacgaacg-3’

 p16-UM_F

5′-ttattagagggtggggtggattgt-3’

 p16-UM_R

5′-caaccccaaaccacaaccataa-3’

 p16-M_F

5′-ttattagagggtggggcggatcgc-3’

 p16-M_R

5′-accccgaaccgcgaccgtaa-3’

Primer sets for MIF polymorphism detection

 rs755622_F

5′-tctagccgccaagtggagaaca-3’

 rs755622_R

5′-actgtggtcccgccttttgtga-3’

 rs5844572_F

5′-tgatccagttgctgccttgtc-3’

 rs5844572_R

5′-tccactaatggtaaactcggggac-3’

  1. MSP Methylation specific RCR, UM Unmethylated, M Methylated, F Forward, R Reverse