Fig. 3
From: Identification and analysis of KLF13 variants in patients with congenital heart disease
KLF13 variants have effects on genetic interaction with TBX5. a: Luciferase activity in NIH3T3 cells cotransfected with wild-type KLF13 or mutant-expression constructs and the BNP promoter, showing that the P163S mutant could decrease the synergistic activation of the BNP promoter, while the S156N mutant increased the synergistic activation of BNP.b: Coimmunoprecipitations were carried out using 293 T cells expressing Myc-KLF13 and TBX5 proteins in combination. The top panel shows Western blots of the nuclear extracts used, with the indicated antibodies. The bottom panel is a Western blot of the anti-TBX5 immunoprecipitates. (*p < 0.05, Statistical significant vs WT, # p < 0.05, Statistical significant vs WT co-transfected with TBX5)