Fig. 2From: Identification and analysis of KLF13 variants in patients with congenital heart diseaseCharacterization of KLF13 variants. a: Immunocytochemical staining to detect the cellular localization of KLF13 variants transfected in 293 T cells. The green color shows the Alexa 488 conjugated secondary antibodies against the anti-myc antibody. The nuclei were visualized with DAPI. b: Western blotting showing the expression of the KLF13 variants. c: The transcriptional activities of KLF13 variants on BNP promoter. Transfections were carried out using the human B-type natriuretic peptide (BNP) promoter and increasing dosages (25, 50, 100, 200, and 300 ng) of expression vectors. Each dosage was conducted in duplicate and the experiment was conducted twiceBack to article page