Table 3 Primer sequences and PCR conditions for the real-time PCR of AIRE mRNA
From: Autoimmune Polyglandular Syndrome Type 1: a case report
mRNA name | Primer sequence (5′ → 3′) | Product size (bp) | Annealing temperature (°C) |
|---|---|---|---|
AIRE | F: CACGACTCTTGTCTACAAGC R: AGGAGCCAGGTTCTGCT | 124 | 60 |
HPRT | F: CCTGGCGTCGTGATTAGTGAT R: AGACGTTCAGTCCTGTCCATAA | 131 | 60 |