Table 1 Primer sequences and positions of single-tube multiplex PCR amplification of PAH gene fragments
From: Rapid detection of PAH gene mutations in Chinese people
Primer name | Sequence (5′-3′) | Detection site | Length (bp) |
|---|---|---|---|
PAHF1 | GCTAGCCTGCCTGCTCT | R1 11X | 145 |
PAHR1 | GAAGACAGTGTGGAGTTACTT | ||
PAHF2 | TGCCCTGCTTGAGACACC | R176X, Ex6–96A > G | 191 |
PAHR2 | CATGGAAGCCACAGTAC TT | ||
PAHF3 | CTAGCGTCAAAGCCTATGT | R241C, R243Q, R252Q | 338 |
PAHR3 | ATGAACCCAAACCTCATTC | ||
PAHF4 | TATGGGATGCAGCAGGG | Y356X, V399 V | 274 |
PAHR4 | ACCACCCACAGATGAGTGG | ||
PAHF5 | TTCTCCAAATGGTGCCCTT | R413P | 232 |
PAHR5 | GCGATGGTAGGGAAAGACAG |