Table 2 PCR primers and conditions used for mutation analysis of the tyrosinase gene
From: Genetic analyses of oculocutaneous albinism types 1 and 2 with four novel mutations
Exon | Sequence(5′-3′) |  |  |  |
|---|---|---|---|---|
|  | Forword | Reverse | Product size(bp) | Annealing temperature(°C) |
1 | TGCTGGAGGTGGGAGTGGTATT | AAATACAAGATACATTGAGAGT | 864 | 56 |
2 | ATTTCTGCCTTCTCCTAC | TAGGACTTTGGATAAGAGACTGTAA | 354 | 56 |
3 | CCAGAATGTAAAGAGTCTCAATACGGAA | TCACAGACAATAGACTACCATAACT | 421 | 56 |
4 | TATGTACCACTTAACTGTG | ACTGCTCTTCACATGGTTGC | 2269 | 62 |
5 | GCCTTCAAACCCAGGTGTCTAC | CACATACAAATAACAGTTCCTC | 455 | 56 |