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Table 1 PCR protocol for HPV detection with SPF primers set

From: Lack of association between methylenetetrahydrofolate reductase C677T polymorphism, HPV infection and cervical intraepithelial neoplasia in Brazilian women

Reagents

Protocol

PCR Master Mix®a

12.5 μl

Primersb (10 pmol/μl)

0.5 μl (each)

Sample (DNA)

25 ng

Final volume

25 μl

  1. a0.2 mM each deoxyribonucleotide (dNTP), 1.5 mM MgCl2 and 1.0 unit of Taq DNA (Promega, Madison, Wisconsin, United States of America)
  2. bNucleotide sequences (5′-3′): SPF 1A: GCiCAGGGiCACAATAATGG; SPF 1B: GCiCAGGGiCATAACAATGG; SPF 1C: GCiCAGGGiCATAATAATGG; SPF 1D: GCiCAAGGiCATAATAATGG; SPF 2B: GTiGTATCiACAACAGTAACAAA; SPF 2D: GTiGTATCiACTACAGTAACAAA