Reagents | Protocol |
---|
PCR Master Mix®a | 12.5 μl |
Primersb (10 pmol/μl) | 0.5 μl (each) |
Sample (DNA) | 25 ng |
Final volume | 25 μl |
- a0.2 mM each deoxyribonucleotide (dNTP), 1.5 mM MgCl2 and 1.0 unit of Taq DNA (Promega, Madison, Wisconsin, United States of America)
- bNucleotide sequences (5′-3′): SPF 1A: GCiCAGGGiCACAATAATGG; SPF 1B: GCiCAGGGiCATAACAATGG; SPF 1C: GCiCAGGGiCATAATAATGG; SPF 1D: GCiCAAGGiCATAATAATGG; SPF 2B: GTiGTATCiACAACAGTAACAAA; SPF 2D: GTiGTATCiACTACAGTAACAAA