Fig. 1

HIF1A driven response in normoxia and in hypoxia conditions. a HIF1A depletion in SKNBE2 was verified by western blotting. The silencing was madiated by two short hairpin against HIF1A (shHIF1A#A and shHIF1A#B). Unsilenced cells were used as control (shCTR). SKNBE2 shHIF1#B was used for RNA sequencing experiments. b The differentially expressed genes in shHIF1A NX vs shCTR NX and in shHIF1A HYP vs shCTR HYP gene sets were crossed and three gene lists were obtained: genes regulated “exclusively in normoxia”, genes regulated “exclusively in hypoxia” and HIF1A target genes. The number of genes for each gene list is reported in the graph. c The Log2 expression of HIF1A target genes is reported for each gene set. d KEGG pathway analysis (webGestalt) of the three gene lists is shown. The negative Log₁₀ pvalue is reported on X-axis (FDR ≤ 0.05). e The reliability of RNAseq data was estimated by assessing the expression values of chosen genes by RT-PCR in SKNBE2 shHIF1A#A and shCTR cells. Log₂Fold of expression in RT-PCR and RNAseq experiments is reported