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Fig. 2 | BMC Medical Genetics

Fig. 2

From: A functional variant alters binding of activating protein 1 regulating expression of FGF7 gene associated with chronic obstructive pulmonary disease

Fig. 2

The risk allele of the variant binds AP-1 subunits with reduced affinity. a A representative figure from three independent western blotting experiments demonstrated the expression of the AP-1 subunits, c-Fos, and c-Jun in fibroblast cells. EMSA revealed that multiple nuclear protein complexes bind to the variant region in which the risk allele of the variant results in reduced binding of the AP-1 subunits. b and c We performed densitometric quantification of AP-1 subunits binding in independent experiments for c-Fos (b) and c-Jun cells (c). Statistical comparisons were made using paired Student’s t-test. d and e ChIP-qPCR was performed using fibroblast cell lines carrying different genotypes of the variants (AA, AG, and GG). ChIP was performed with antibodies specific against c-Fos and c-Jun subunits, respectively, followed by qPCR with primers neighboring the variant rs12905203 region. Statistical comparisons were made using one-way ANOVA

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