Table 2 SNP PCR primers
SNP | Primer | Size (bp) | Tm (°C) |
|---|---|---|---|
rs1024161 | F:AGAAATTTGAGTTAAAGGCTCT R:GAATGTACAGATAATGTCACTCT | 102 | 53 |
rs5742909 | F:CTCCAAGTCTCCACTTAGTT R:GAAGCTTCATGTTCACTTT | 51 | 53 |
rs231775 | F:CCTTGGATTTCAGCGGCAC R:AGAGTGCAGGGCCAGGT | 63 | 59.7 |
rs231777 | F:ATTGAATCTCAACCTTATCTCTCTC R:ACCTACTTCATACAAACTACATGG | 70 | 56.2 |
rs231779 | F:TGCAGCCACTATTTTTGAGTTGA R:ACACTCCCATGCTCCTTTGT | 96 | 59.2 |
rs3087243 | F:TTCACCACTATTTGGGATATAA R:GTGTTAAACAGCATGCCAAT | 80 | 53 |
rs11571319 | F:TGGGTTAACACAGACATA R:CCTGTGTTAAACAGCATGCCA | 59 | 50.5 |
rs11571302 | F:ATGGGTTGTTCCACGACTTC R:AAACGCTGCCAATAAACAGTC | 76 | 57 |
rs11571297 | F:TTACTTTTAACTTCCATTCCCAGC R:TCTACCAGAAGTTGAAGTGTAGG | 82 | 57.3 |
rs1883832 | F:CTGCCGCCTGGTCTCAC R:ACTGCAGAGGCAGACGAAC | 45 | 60 |