- Research article
- Open Access
- Open Peer Review
Effect of a rare genetic variant of TM7SF4 gene on osteoclasts of patients with Paget’s disease of bone
© The Author(s). 2017
- Received: 3 August 2017
- Accepted: 9 November 2017
- Published: 16 November 2017
Dendritic Cell-Specific Transmembrane Protein (DC-STAMP) is involved in osteoclastogenesis with a key role in mononucleated osteoclasts fusion. We reported in patients with Paget’s disease of bone (PDB) a rare variant (rs62620995) in the TM7SF4 gene, encoding for DC-STAMP, which changes a highly conserved amino acid, possibly damaging according to in silico predictions. This study aimed at determining the functional effects of this variant on osteoclast phenotype in PDB.
Fifty ml of peripheral blood were collected in pagetic patients carrier of this variant (n = 4) or not (n = 4) and healthy controls (n = 4). Monocytes were collected after Ficoll gradient and cultured in a medium containing RANKL (40 ng/ml) and hMCSF (25 ng/ml). At the end of the differentiation period, we assessed the osteoclast morphology and bone resorption abilities. We quantified gene expression of SQSTM1, DC-STAMP, OS9, CREB3, LAMP1, OC-STAMP, and NFATC1 genes from cell lysates. Proteins encoded by these genes were investigated by Western Blot. Statistical analyses relied on ANOVA followed by Tukey post-tests.
After 21 days of differentiation, the mean number of nuclei per multinucleated cell was significantly higher in pagetic patients carrier of the variant than in healthy controls. Bone resorption abilities were not modified by the variant. qPCR and Western Blot analyses did not provide any differences, but DC-STAMP expression was higher in patients carrier of the variant than in patients non carrier.
This TM7SF4 rare variant may have an impact on osteoclast morphology and on DC-STAMP expression during osteoclastogenesis. Further analyses are required to understand the role of this variant during osteoclastogenesis in PDB.
- Paget’s disease of bone
- TM7SF4 gene
- DC-STAMP protein
- Rare genetic variant
Paget’s disease of bone (PDB) is a common metabolic bone disorder affecting up to 3% of the Caucasian population after the age of 55 and more than 10% after 80 years of age . This disease is characterized by overactive osteoclasts leading to increased bone resorption followed by a coupled excessive bone formation by osteoblasts . Although every bone may be affected by PDB, vertebrae, skull, pelvis, femur and tibia are the most frequently affected bones. PDB symptoms, which vary depending on the affected bones and the severity of the disease, may go from joint pain to fractures, bone deformity, bone pain, headache, and deafness . The pathophysiology of PDB is not well-understood yet, but environmental, viral and genetic factors are likely to be involved [2, 4]. Fifteen to 30% of patients with PDB have a family history of this disease, which display an autosomal mode of inheritance with high but incomplete penetrance [5–7]. Sequestosome 1 (SQSTM1) gene mutations are frequently reported in familial forms of the disease as well as in 10 to 15% of non familial cases [7, 8]. In addition, genome-wide association studies (GWAS) in European patients with PDB identified significant associations of PDB with common genetic variants (SNPs) located in 1p13 (near CSF1 gene), in 7q33 (NUP205 gene), in 8q22 (TM7SF4), in 10p13 (OPTN), in 14q32 (RIN3) and in 15q24 (PML) [9–11].
In the French Canadian population, although no allelic association was found with the rs2458413, an intron variant in TM7SF4 gene (locus 8q22) previously identified in GWAS, a genotypic association was found for this common variant for the heterozygous genotype AG versus both homozygous genotypes AA and GG (uncorrected p = 6.9 × 10−4; RR = 1.73 [1.22–2.45]) . Further sequencing candidate genes in novel PDB-associated loci [9–11] allowed our team to identify several rare variants with potential functional effects on PDB phenotypes, in particular a rare variant, rs62620995, located in the fifth exon of the TM7SF4 gene . In the subgroup of our cohort consisting in pagetic patients non carrier of SQSTM1 gene mutations, the minor allele frequency of the T allele of this variant was twice more frequent in patients with PDB than healthy controls (2.8% versus 1.4%, p = 0.09, OR = 2.06 [0.85–5.01]). The global distribution of the rs6262099 variant genotypes differed between patients and controls (p = 0.044). 5.6% of patients were heterozygous CT versus 2.7% of healthy controls, whereas no homozygous TT was found in both groups. The presence of at least one major allele (C) in the genotype suggested a protective effect against PDB .
The TM7SF4 gene encodes the DC-STAMP protein (470 amino acids; 53,393 Da), a transmembrane protein involved in osteoclast multinucleation and osteoclastogenesis . The rare genetic variant rs62620995 leads to a non-synonymous change p. Leu 397 Phe located in the seventh and last transmembrane domain of the protein. The leucine being highly conserved in the evolution  leads us to believe that this amino acid could have an important role in the DC-STAMP protein function. This change was in silico predicted to be damaging (Polyphen, Condel) as it modify an amino-acid located in one of the transmembrane domains of the protein , which are critical for this receptor internalization .
Since hypermultinucleation is an important characteristic of pagetic osteoclasts, this non synonymous coding rare variant, rs62620995, could contribute to PDB pathophysiology. In this study, we aimed at studying the functional consequences of this variant on osteoclast phenotype and bone resorption abilities, as well as on gene and protein expressions in in vitro differentiated osteoclasts from patients with PDB carriers and non-carriers and healthy controls.
Recruitment of participants
Phenotype assessment of our French Canadian cohort comprised a complete bone evaluation, including total serum alkaline phosphatase, a total body bone scan and skull and pelvis X-rays, as previously reported [12, 16–18]. For all participants, SQSTM1 gene mutations were searched by Sanger sequencing and rs62620995 was genotyped by Sequenom analysis, as previously reported [7, 12].
In vitro differentiation of monocytes from the peripheral blood into mature osteoclasts
Main clinical characteristics of study participants
Age range (y)
Age at diagnosis (y)
Renier’s index (%)
Number of affected bones
Details of involved bones
CT for rs62620995
Skull, face excluding the mandibula
Sacrum, right and left pelvis, left scapula, both humeri, 3 consecutive lumbar vertebrae
CT for rs62620995
Left humerus, D8, D12, right pelvis, and both femurs
CT for rs62620995
CT for rs62620995
Left proximal femur, left tibia
2/3 of left femur
Immunofluorescence of DC-STAMP protein
Osteoclasts were rinsed with PBS, fixed with paraformaldehyde 4%, rinsed again 3 times for 5 min with PBS and incubated 20 min with PBS 10% FBS. Then they were incubated 60 min with DC-STAMP antibody, 1: 100 in PBS 1.5% FBS (Santa Cruz Biotechnology # sc-87,673, polyclonal rabbit IgG) and rinsed 3 times with PBS. Osteoclasts were also incubated 45 min with anti-rabbit IgG ALEXA Fluor 594, 1: 500 in PBS 1.5% FBS (Cell Signaling 8889S) and rinsed 3 times with PBS. Cells were incubate 3 min with PBS-DAPI (0.3 μM final) (Molecular Probes D1306), rinsed 3 times with PBS and covered with PBS-glycerol 1: 1. Pictures were taken in fluorescence at 40X.
Quantitative real-time PCR
Sequence primers and gene description
Primer sequence 5′ → 3′
Homo sapiens sequestosome 1 (SQSTM1), region common to the 3 transcripts
Homo sapiens dendrocyte expressed seven transmembrane protein (DCSTAMP)
Homo sapiens osteosarcoma amplified 9, endoplasmic reticulum lectin (OS9), 8 transcripts
Homo sapiens cAMP responsive element binding protein 3
Homo sapiens lysosomal associated membrane protein 1 (LAMP1)
Homo sapiens osteoclast stimulatory transmembrane protein (OCSTAMP)
Homo sapiens nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATC1),7 transcripts
Homo sapiens glucose-6-phosphate dehydrogenase (G6PD), nuclear gene encoding mitochondrial protein
Homo sapiens peptidylprolyl isomerase B (cyclophilin B) (PPIB)
Homo sapiens 18S ribosomal RNA
Homo sapiens 3-beta-hydroxysteroid dehydrogenase/delta-5-delta-4-isomerase (3-beta-HSD) gene (intron)
Western blot analyses
Cell lysates were separated by SDS-PAGE, and transferred to a PVDF membrane. The membrane was incubated with the primary antibodies. We used a primary antibody against DC-STAMP, OC-STAMP, SQSTM1, LAMP1, OS-9 and CREB3 also known as Luman, and an anti-alpha tubulin or vinculin antibody as a loading control. HRP-conjugated secondary antibodies were used to achieve detection with chemiluminescent system. Protein expression was quantified by densitometry using the Molecular Imager Gel Doc XR and Imaging System.
We compared osteoclast phenotype characteristics, gene and protein expressions in three groups consisting in PDB patients (with or without the rs62620995 rare variant) and from healthy donors non carrier of this variant. Statistical analyses, performed by the use of GraphPadPrism, relied on ANOVA followed by Tukey post-tests. P-values <0.05 were considered statistically significant.
Osteoclast morphology and bone resorption abilities
Immunofluorescence of the DC-STAMP protein
Gene expression analyses from cell lysates of in vitro differentiated osteoclasts
Protein expression analysis by western blot in cell lysates of in vitro differentiated osteoclasts
In comparison to healthy controls, DC-STAMP expression was lower in patients non carrier of the variant but not in patients carrier of this rare variant. SQSTM1 and OS-9 expressions were lower in patients than in healthy controls, in particular in patients non carrier of the rare variant for isoforms 1 and 2 of OS-9 which were more than twice lower than in healthy controls (see the left part of the bottom line of Fig. 4). Patients carrier of the rare variant had the highest mean level of expression of CREB3 in comparison to patients non carrier of the variant and healthy controls.
In this study, we investigated the functional effects of a rare genetic variant, rs62620995, within TM7SF4 gene at locus 8q22, on osteoclast phenotype, as well as on gene and protein expressions in in vitro differentiated osteoclasts from patients with PDB, carrier of this rare variant or not, and healthy controls. We found that osteoclasts differentiated in vitro for 21 days from patients carrier of this variant significantly yielded to a higher number of nuclei per cell in comparison to healthy controls, suggesting a faster cell fusion in pagetic patients carrier of the variant. Although gene expression analyses and Western Blots did not provide any significant results, we observed that DC-STAMP expression was similar in patients carrier of the variant than in healthy controls which was unexpected at the end of the differentiation period of 21 days. Indeed, DC-STAMP, like other fusion regulators such as DAP12/FcRγ, TRAF6, NFATc1, MITF, CD47/TSP1, ATP6v0d2, CD44 and ADAM8/α9β1-integrin, is mainly expressed in committed pre-osteoclast as a critical player for fusion into multinucleated osteoclast . But after the cell fusion which is known to occur earlier during the osteoclastogenesis of pagetic osteoclasts than in healthy osteoclasts, DC-STAMP expression is expected to rapidly decrease. The persistence of a relative high expression of DC-STAMP in osteoclasts from patients carrier of the variant may suggest a possible effect of the rare variant on DC-STAMP protein stability. The overexpression of CREB3, also known as Luman, in osteoclasts from patients carrier of the variant is interesting since this transcription factor is known to regulate the multinucleation of osteoclasts by promoting mononuclear osteoclasts fusion through DC-STAMP induction and intracellular distribution during osteoclastogenesis . Our results of immunofluorescence on mature osteoclasts shown intracellular internalized localization of DC-STAMP which was already reported in a fraction of RANKL-induced osteoclast precursors whereas a localization to the cell surface was also detected in osteoclasts and undifferentiated monocytes . DC-STAMP is known to interact via the C-terminus cytoplasmic tail with both isoforms of OS-9, which is one of lectin that regulates endoplasmic reticulum-associated degradation and quality control of protein folding [29, 30]. This interaction with OS-9 isoform 1 results in a redistribution of DC-STAMP to the endoplasmic reticulum and Golgi intermediate compartment.
According to the literature, the osteoclast phenotype in PDB was reported to have several characteristics such as an increased osteoclast number, an increased number of nuclei per osteoclast, an increased bone resorption capacity per osteoclast, an increased responsitivity to 1,25-(OH)2 vitamin D3, RANKL and TNF, an increased expression of TAF12 and an increased production of IL6 per osteoclast . To date, no genetic variant linked to PDB gave rise to the full osteoclast phenotype, as cited above. In vitro, the p.Pro392Leu mutation within SQSTM1 gene, which is the most frequent mutation found in patients with PDB, transfected in osteoclast precursors lead to hyperresponsitivity to RANKL, TNF but not 1,25-(OH)2 vitamin D3. Although the number of osteoclast was increased in the mouse model expressing this mutation, the number of nuclei per osteoclast was not found to be increased [31, 32]. To date, the most complete pagetic phenotype of osteoclasts was reported in vitro and in vivo by the expression of the measles virus nucleocapsid gene .
DC-STAMP role in osteoclastogenesis and during the late stage of cell fusion has been intensively studied since the first publication of its role in osteoclast in 2004 . A mouse model of DC-STAMP deficient mice was generated, displaying major defects in osteoclast multinucleation which reduce bone resorption, resulting in a phenotype of osteopetrosis. Although a common variant of TM7SF4 gene was found to be associated to PDB in 2010 [9, 10], no functional studied of DC-STAMP in PDB have been published yet.
The small number of samples studied here, due to the rarity of this genetic variant in our French-Canadian cohort, may have limited the statistical power to demonstrate differences between groups. Moreover, we found that the global area of resorbed bone was similar in the three groups and the ratio of resorbed bone per percentage of multinucleated cell was the highest in healthy controls. This observation may be due to the concentration of 40 ng/ml of RANKL chosen for this project, which was enough for osteoclastogenesis but possibly not enough to stimulate pagetic osteoclast bone resorption. In accordance to the hyperresponsitivity to RANKL of pagetic osteoclasts, stimulation of bone resorption with higher concentration of RANKL up to 100 ng/ml was previously reported in in vitro differentiated human osteoclasts in PDB .
The functional effect of this rare genetic variant in TM7SF4 gene should be further investigated in several ways, in PDB as well as in other bone disorders such as osteoporosis, giant cell tumors or osteopetrosis. DC-STAMP appears more and more in the literature as a possible novel therapeutic target for periodontal diseases , as a biomarker for psoriatic arthritis or as a way to deliver drugs in bone fractures sites . In vitro studies should be undertaken to investigate the role of the rare genetic variant on the expression of DC-STAMP at different times during osteoclastogenesis and under different conditions such as with a gradient of concentration of RANKL or by the use of cocultures of osteoclast precursors with stromal cells to reproduce more physiological conditions like in the bone microenvironment. The folding of the protein and the impact of the rare variant, located in the seventh and last transmembrane domain of the protein, on the Immunoreceptor Tyrosine-based Inhibitory Motif (ITIM)-mediated signaling of the cytoplasmic tail of DC-STAMP should also be further studied .
Our results suggest that the rare genetic variant of TM7SF4 gene, found in our French-Canadian cohort of patients with PDB and which encodes the DC-STAMP protein, increase the number of nuclei per multinucleated cells and affect DC-STAMP expression during osteoclastogenesis in PDB. Further analyses will help at better understanding the role of this rare genetic variant on osteoclast morphology and osteoclastogenesis.
The authors would like to acknowledge the CHU de Québec Research Centre (CHUL) Gene Expression Platform, Quebec, Canada for the quantitative real-time PCR measurements.
Dr. Michou is supported by a career award from the Fonds de recherche du Québec-santé (FRQ-S). This study was funded by Canadian Institutes for Health Research (MOP130457), Canada, CHU de Québec Foundation, Canadian Foundation for Innovation, Fonds de recherche du Québec-santé, Laval University and CHU de Québec Research Centre.
Availability of data and materials
All data generated or analysed during this study are included in this published article [and its supplementary information files].
Study design: EL, NA, LM. Patient recruitment and acquisition of data: EL, NA, EG, JPB, LM. Analysis and interpretation of data: EL, NA, EG, JPB, LM. Revision of manuscript content: all authors. Approving final version of manuscript: all authors.
Ethics approval and consent to participate
The present study was approved by the CHU de Québec-Université Laval Ethics Committee (project number 2014–1820, B13–11-1820). After information on the study, all individuals signed a consent form before recruitment.
Consent for publication
The authors declare that they have no competing interests relating to the contents of the manuscript. JPB has received consulting fees from Amgen Inc., Eli Lilly and Merck, and has received research grants from and has served on speakers’ bureau for Amgen Inc. and Eli Lilly.
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- van Staa TP, Selby P, Leufkens HG, Lyles K, Sprafka JM, Cooper C. Incidence and natural history of Paget's disease of bone in England and Wales. J Bone Miner Res. 2002;17(3):465–71.View ArticlePubMedGoogle Scholar
- Galson DL, Roodman GD. Pathobiology of Paget's disease of bone. Journal of bone metabolism. 2014;21(2):85–98.View ArticlePubMedPubMed CentralGoogle Scholar
- Michou L, Brown JP. Paget's disease of bone: prognosis and complications. Advances in Pathobiology and Management of Paget’s Disease of Bone SV Reddy (Ed). 2016:105–18.Google Scholar
- Singer FR. Paget's disease of bone-genetic and environmental factors. Nat Rev Endocrinol. 2015;11(11):662–71.View ArticlePubMedGoogle Scholar
- Morales-Piga AA, Rey-Rey JS, Corres-Gonzalez J, Garcia-Sagredo JM, Lopez-Abente G. Frequency and characteristics of familial aggregation of Paget's disease of bone. J Bone Miner Res. 1995;10(4):663–70.View ArticlePubMedGoogle Scholar
- Siris ES, Ottman R, Flaster E, Kelsey JL. Familial aggregation of Paget's disease of bone. J Bone Miner Res. 1991;6(5):495–500.View ArticlePubMedGoogle Scholar
- Morissette J, Laurin N, Brown JP. Sequestosome 1: mutation frequencies, haplotypes, and phenotypes in familial Paget's disease of bone. J Bone Miner Res. 2006;21(Suppl 2):P38–44.View ArticlePubMedGoogle Scholar
- Chung PY, Van Hul W. Paget's disease of bone: evidence for complex pathogenetic interactions. Semin Arthritis Rheum. 2012;41(5):619–41.View ArticlePubMedGoogle Scholar
- Albagha OM, Visconti MR, Alonso N, Langston AL, Cundy T, Dargie R, Dunlop MG, Fraser WD, Hooper MJ, Isaia G, et al. Genome-wide association study identifies variants at CSF1, OPTN and TNFRSF11A as genetic risk factors for Paget's disease of bone. Nat Genet. 2010;42(6):520–4.View ArticlePubMedPubMed CentralGoogle Scholar
- Chung PY, Beyens G, Boonen S, Papapoulos S, Geusens P, Karperien M, Vanhoenacker F, Verbruggen L, Fransen E, Van Offel J, et al. The majority of the genetic risk for Paget's disease of bone is explained by genetic variants close to the CSF1, OPTN, TM7SF4, and TNFRSF11A genes. Hum Genet. 2010;128(6):615–26.View ArticlePubMedGoogle Scholar
- Albagha OM, Wani SE, Visconti MR, Alonso N, Goodman K, Brandi ML, Cundy T, Chung PY, Dargie R, Devogelaer JP, et al. Genome-wide association identifies three new susceptibility loci for Paget's disease of bone. Nat Genet. 2011;43(7):685–9.View ArticlePubMedGoogle Scholar
- Beauregard M, Gagnon E, Guay-Belanger S, Morissette J, Brown JP, Michou L. Identification of rare genetic variants in novel loci associated with Paget's disease of bone. Hum Genet. 2014;133(6):755–68.View ArticlePubMedGoogle Scholar
- Yagi M, Miyamoto T, Sawatani Y, Iwamoto K, Hosogane N, Fujita N, Morita K, Ninomiya K, Suzuki T, Miyamoto K, et al. DC-STAMP is essential for cell-cell fusion in osteoclasts and foreign body giant cells. J Exp Med. 2005;202(3):345–51.View ArticlePubMedPubMed CentralGoogle Scholar
- Hartgers FC, Vissers JL, Looman MW, van Zoelen C, Huffine C, Figdor CG, Adema GJ. DC-STAMP, a novel multimembrane-spanning molecule preferentially expressed by dendritic cells. Eur J Immunol. 2000;30(12):3585–90.View ArticlePubMedGoogle Scholar
- Mensah KA, Ritchlin CT, Schwarz EM. RANKL induces heterogeneous DC-STAMP(lo) and DC-STAMP(hi) osteoclast precursors of which the DC-STAMP(lo) precursors are the master fusogens. J Cell Physiol. 2010;223(1):76–83.PubMedPubMed CentralGoogle Scholar
- Beauregard M, Gagnon E, Guay-Belanger S, Siris ES, Morissette J, Brown JP, Michou L. Genetic association study of Dickkopf-1 and sclerostin genes with paget disease of bone. Calcif Tissue Int. 2013;93(5):405–12.View ArticlePubMedGoogle Scholar
- Guay-Belanger S, Picard S, Gagnon E, Morissette J, Siris ES, Orcel P, Brown JP, Michou L. Detection of SQSTM1/P392L post-zygotic mutations in Paget's disease of bone. Hum Genet. 2015;134(1):53–65.View ArticlePubMedGoogle Scholar
- Laurin N, Brown JP, Morissette J, Raymond V. Recurrent mutation of the gene encoding sequestosome 1 (SQSTM1/p62) in Paget disease of bone. Am J Hum Genet. 2002;70(6):1582–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Filgueira L. Fluorescence-based staining for tartrate-resistant acidic phosphatase (TRAP) in osteoclasts combined with other fluorescent dyes and protocols. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 2004;52(3):411–4.View ArticleGoogle Scholar
- Theriault P, ElAli A, Rivest S. High fat diet exacerbates Alzheimer's disease-related pathology in APPswe/PS1 mice. Oncotarget. 2016;7(42):67808–27.View ArticlePubMedPubMed CentralGoogle Scholar
- Aubin K, Safoine M, Proulx M, Audet-Casgrain MA, Cote JF, Tetu FA, Roy A, Fradette J. Characterization of in vitro engineered human adipose tissues: relevant Adipokine secretion and impact of TNF-alpha. PLoS One. 2015;10(9):e0137612.View ArticlePubMedPubMed CentralGoogle Scholar
- Luu-The V, Paquet N, Calvo E, Cumps J. Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction. BioTechniques. 2005;38(2):287–93.View ArticlePubMedGoogle Scholar
- Warrington JA, Nair A, Mahadevappa M, Tsyganskaya M. Comparison of human adult and fetal expression and identification of 535 housekeeping/maintenance genes. Physiol Genomics. 2000;2(3):143–7.PubMedGoogle Scholar
- Bustin SA, Beaulieu JF, Huggett J, Jaggi R, Kibenge FS, Olsvik PA, Penning LC, Toegel S. MIQE precis: practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments. BMC Mol Biol. 2010;11:74.View ArticlePubMedPubMed CentralGoogle Scholar
- Bustin SA, Benes V, Garson JA, Hellemans J, Huggett J, Kubista M, Mueller R, Nolan T, Pfaffl MW, Shipley GL, et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem. 2009;55(4):611–22.View ArticlePubMedGoogle Scholar
- Galson DLR. G.D.: origin of osteoclasts. Osteoimmunology Interactions of the Immune and Skeletal Systems Edited by:Joseph Lorenzo, MD, Yongwon Choi, PhD, Mark Horowitz. MD PhD: PhD, and Hiroshi Takayanagi; 2011. p. 7–41.Google Scholar
- Kanemoto S, Kobayashi Y, Yamashita T, Miyamoto T, Cui M, Asada R, Cui X, Hino K, Kaneko M, Takai T, et al. Luman is involved in osteoclastogenesis through the regulation of DC-STAMP expression, stability and localization. J Cell Sci. 2015;128(23):4353–65.View ArticlePubMedPubMed CentralGoogle Scholar
- Kukita T, Wada N, Kukita A, Kakimoto T, Sandra F, Toh K, Nagata K, Iijima T, Horiuchi M, Matsusaki H, et al. RANKL-induced DC-STAMP is essential for osteoclastogenesis. J Exp Med. 2004;200(7):941–6.View ArticlePubMedPubMed CentralGoogle Scholar
- Hosokawa N, Kamiya Y, Kamiya D, Kato K, Nagata K. Human OS-9, a lectin required for glycoprotein endoplasmic reticulum-associated degradation, recognizes mannose-trimmed N-glycans. J Biol Chem. 2009;284(25):17061–8.View ArticlePubMedPubMed CentralGoogle Scholar
- Roth J, Zuber C. Quality control of glycoprotein folding and ERAD: the role of N-glycan handling, EDEM1 and OS-9. Histochem Cell Biol. 2017;147(2):269–84.View ArticlePubMedGoogle Scholar
- Kurihara N, Hiruma Y, Zhou H, Subler MA, Dempster DW, Singer FR, Reddy SV, Gruber HE, Windle JJ, Roodman GD. Mutation of the sequestosome 1 (p62) gene increases osteoclastogenesis but does not induce Paget disease. J Clin Invest. 2007;117(1):133–42.View ArticlePubMedGoogle Scholar
- Daroszewska A, Hof RJ V’t, Rojas JA, Layfield R, Landao-Basonga E, Rose L, Rose K, Ralston SH. A point mutation in the ubiquitin-associated domain of SQSMT1 is sufficient to cause a Paget's disease-like disorder in mice. Hum Mol Genet. 2011;20(14):2734–44.View ArticlePubMedGoogle Scholar
- Kurihara N, Hiruma Y, Yamana K, Michou L, Rousseau C, Morissette J, Galson DL, Teramachi J, Zhou H, Dempster DW, et al. Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease. Cell Metab. 2011;13(1):23–34.View ArticlePubMedPubMed CentralGoogle Scholar
- Chamoux E, Couture J, Bisson M, Morissette J, Brown JP, Roux S. The p62 P392L mutation linked to Paget's disease induces activation of human osteoclasts. Mol Endocrinol. 2009;23(10):1668–80.View ArticlePubMedPubMed CentralGoogle Scholar
- Wisitrasameewong W, Kajiya M, Movila A, Rittling S, Ishii T, Suzuki M, Matsuda S, Mazda Y, Torruella MR, Azuma MM, et al. DC-STAMP is an osteoclast Fusogen engaged in periodontal bone resorption. J Dent Res. 2017;96(6):685–93.View ArticlePubMedGoogle Scholar
- Chiu YH, Ritchlin CT. DC-STAMP: a key regulator in osteoclast differentiation. J Cell Physiol. 2016;231(11):2402–7.View ArticlePubMedPubMed CentralGoogle Scholar
- Chiu YH, Schwarz E, Li D, Xu Y, Sheu TR, Li J, de Mesy Bentley KL, Feng C, Wang B, Wang JC, et al. Dendritic cell-specific transmembrane protein (DC-STAMP) regulates osteoclast differentiation via the Ca2+ /NFATc1 Axis. J Cell Physiol. 2017;232(9):2538–49.View ArticlePubMedGoogle Scholar