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Table 1 Primers, restriction enzymes, and fragment lengths of the major and minor alleles of rs1799983, rs2070744, and VNTR polymorphisms of NOS3 gene

From: Association of NOS3 gene polymorphisms with essential hypertension in Sudanese patients: a case control study

 

rs1799983

VNTR intron 4

rs2070744

Left primer (5′ to 3′)

AGCCTCGGTGAGATAAAGGA

AGGCCCTATGGTAGTGCCTT

CCCCTGTGGACCAGATGC

Right primer (5′ to 3′)

TCTTGAGAGGCTCAGGGATG

TCTCTTAGTGCTGTGGTCAC

ACATTAGGGTATCCCTTCC

Product size

368 bp

 

379 bp

Restriction enzyme

BanII

MboI

 

MspI

Major allele fragment length (bp)

Cut, two fragments (251 and 117 bp)

Not cut

420 (b)

Two fragments, 146 and 233 bp

Minor allele fragment length (bp)

Not cut, 368 bp

Cut, two fragments (246 and 122 bp)

394 (a)

Three fragments 46, 146, and 187 bp

Heterozygous

  

394 and 420 bp fragments

Four fragments 46, 146, 187, and 233 bp