Table 1 Primer sets used for detecting CFTR gene mutations. The same set of primers was used for detecting mutations I507del and F508del. Primers flank 13 targets and allow the detection of 14 CFTR mutations
From: A simple, fast and inexpensive method for mutation scanning of CFTR gene
CFTR Mutation | Sequence (5′–3′) | Description | Amplicon length (bp) |
|---|---|---|---|
R334W | TTTGTTTATTGCTCCAAGAGAGTCATACCA | antisense common primer | 140 |
CCTATGCACTAATCAAAGGAATCATCCTGC | sense wild type | ||
CCTATGCACTAATCAAAGGAATCATCCTGT | sense mutant | ||
I507del/F508del | GGGTAGTGTGAAGGGTTCATATGCATAAT | antisense common primer | 146 |
GCCTGGCACCATTAAAGAAAATATCATTG | sense mutant | ||
GCCTGGCACCATTAAAGAAAATATCATCT | sense wild type | ||
1717-1G > A | TAAAATTTCAGCAATGTTGTTTTTGACC | sense common primer | 221 |
TGTCTTTCTCTGCAAACTTGGAGATGTTC | antisense wild type | ||
TGTCTTTCTCTGCAAACTTGGAGATGTTT | antisense mutant | ||
G542X | TAAAATTTCAGCAATGTTGTTTTTGACC | sense common primer | 257 |
ACTCAGTGTGATTCCACCTTCTAC | antisense wild type | ||
CACTCAGTGTGATTCCACCTTCTCA | antisense mutant | ||
G551D | TAAAATTTCAGCAATGTTGTTTTTGACC | sense common primer | 285 |
GCTAAAGAAATTCTTGCTCGTTGCC | antisense wild type | ||
AGCTAAAGAAATTCTTGCTCGTTGCT | antisense mutant | ||
R553X | TAAAATTTCAGCAATGTTGTTTTTGACC | sense common primer | 291 |
CACCTTGCTAAAGAAATTCTTGCTAG | antisense wild type | ||
CACCTTGCTAAAGAAATTCTTGCTAA | antisense mutant | ||
1811 + 1,6KbA > G | TAAATTGGCTTTAAAAATTTCTTAATTG | sense common primer | 135 |
CAGGTGTGATTGATAGTAACCTTACTTCT | antisense wild type | ||
CAGGTGTGATTGATAGTAACCTTACTTCC | antisense mutant | ||
2183AA > G | CAGCCAGACTTTAGCTCAAAACTCATGGG | sense common primer | 170 |
AACTCTCCAGTCTGTTTAAAAGATTATT | antisense wild type | ||
AACTCTCCAGTCTGTTTAAAAGATTAC | antisense mutant | ||
3120 + 1G > A | CCTCTTACCATATTTGACTTCATCCACG | sense wild type | 197 |
CCTCTTACCATATTTGACTTCATCCACA | sense mutant | ||
AATTTACTAAACTTATGTCTATTTTGAAGGC | antisense common primer | ||
3272-26A > G | CATATCTATTCAAAGAATGGCACCAGTGT | sense common primer | 165 |
TGCCTGTGAAATATTTCCATAGAAAACGT | antisense wild type | ||
TGCCTGTGAAATATTTCCATAGAAAACGC | antisense mutant | ||
R1162X | TTTTGCTGTGAGATCTTTGACAGTCATTT | antisense common primer | 200 |
TATTTTTATTTCAGATGCGATCTGTGAGTC | sense wild type | ||
TATTTTTATTTCAGATGCGATCTGTGAGTT | sense mutant | ||
W1282X | CCCATCACTTTTACCTTATAGGTGGGCCTC | sense common primer | 178 |
CCTGTGGTATCACTCCAAAGGCTTTCCAC | antisense wild type | ||
CCTGTGGTATCACTCCAAAGGCTTTCCAT | antisense mutant | ||
N1303K | GAGAGAACTTGATGGTAAGTACATGGGTGTTTC | sense common primer | 206 |
GATCACTCCACTGTTCATAGGGATCCAAG | antisense wild type | ||
GATCACTCCACTGTTCATAGGGATCCAAC | antisense mutant |