Fig. 4
From: A simple, fast and inexpensive method for mutation scanning of CFTR gene
Determination of the genotype of the sample in the mutant allele specific multiplex PCR. Melting curves obtained at a ramp rate of 0.01 °C/s. Each color represents a genotype: green (non-carrier), blue (R553X), pink (3120 + 1G > A), brown (F508/N1303K), grey (R334W) and yellow (G542X). HRMA was performed to allow the identification of the mutations when possible