Mutation | Oligo Name | Oligo sequence 5’–3’ | Annealing temperature (°C) |
---|---|---|---|
Exon 8 c.1079G > A;C360Y (hg19 - c.1283G > A; C428Y) | WT1_E8_F _A E8_C428Y_R_C | TTCCCCAAGGTGAGAAACCA ACCTTCGTTCATAGTCCTTGAAG | 56 |
E8_C428Y_F_ B WT1_E8_R_D | CTTCAAGGACTATGAACGAAGGT GCTGCCAGCAATGAGAAGTG | 58 | |
Exon 8 c.1119C > A;H373Q (hg19 - c.1323C > A;H441Q) | WT1_E8_F _A E8_H441Q_R_C | TTCCCCAAGGTGAGAAACCA TGTCTCCTTTGTTGTCTTTTGAG | 58 |
E8_ H441Q _F_ B WT1_E8_R_D | CTCAAAAGACAACAAAGGAGACA GCTGCCAGCAATGAGAAGTG | 58 | |
Exon 9 c.1180C > T;R394W (hg19 - c.1384C > T;R462W) | WT1_E9_F_A E9_R462W_R_C | TTGTTAGGGCCGAGGCTAGA TGGTCGGACCAGGAGAAC | 56 |
E9_R462W_F _B WT1_E9_R _D | GTTCTCCTGGTCCGACCA AGTGCGTAAACTTTTCTTCACAT | 60 | |
Exon 9 c.1190A > C;H397P (hg19 - c.1394A > C; H465P) | WT1_E9_F_A E9_ H465P_R_C | TTGTTAGGGCCGAGGCTAGA GTCTTCAGGGGGTCGGACC | 60 |
E9_ H465_F_B WT1_E9_R _D | GGTCCGACCCCCTGAAGAC AGTGCGTAAACTTTTCTTCACAT | 52 | |
Exon 9 c.A1200C > T;H401Y (hg19 - c.1405C > T;H469Y) | WT1_E9_F_A E9_ H469Y_R_C | TTGTTAGGGCCGAGGCTAGA GTCCTGGTGTGAGTCTTCAGGTG | 58 |
E9_ H469Y_F_B WT1_E9_R _D | CACCTGAAGACTCACACCAGGAC AGTGCGTAAACTTTTCTTCACAT | 56 |