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Fig. 1 | BMC Medical Genetics

Fig. 1

From: Rapid detection of pathological mutations and deletions of the haemoglobin beta gene (HBB) by High Resolution Melting (HRM) analysis and Gene Ratio Analysis Copy Enumeration PCR (GRACE-PCR)

Fig. 1

Schematic representation the HBB gene showing amplicons and probe positions. A total of 11 primer pairs were used to scan the HBB gene by HRM-PCR (H1 to H11). In addition two primer pairs where used to test for deletions by GRACE-PCR (G1 and G2). The unlabelled probe (indicated by P) was used in conjunction with primer set H2. Primer sets H6 and H7 incorporated the universal base inosine in place of common non-pathological SNPs (See sequence information in Table 1)

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BMC Medical Genetics

ISSN: 1471-2350

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