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Table 2 Primers for PCR amplification of CCM genes promoter regions

From: CCM3/SERPINI1 bidirectional promoter variants in patients with cerebral cavernous malformations: a molecular and functional study

 

Primer set

Anneal (°C)

a CCM1

F: 5’ – ACA GAC AGA ACA ACA ATG CTC – 3’

R: 5’ – TCA CTG GAC CTG CAG TCT CT – 3’

58

a CCM2

F: 5’ – TTT ACA TTC TAG CTG TGC TA – 3’

R: 5’ - CTG GAC AGG TGC GTT CTC – 3’

49

CCM3- SERPINI1

F: 5’ - TGAGGCACTGACTTCACTT-3’

R: 5’-CTTAGCTGCTCTCAGGGA-3’ (P1)

58

F: 5’ - TCCCTGAGAGCAGCTAAG-3’

R: 5’ - GCTCTCGTTCCTGCTTTC-3’ (P2)

56

  1. aPrimers were designed to amplify a promoter region located about 3.8 kb (CCM1) and 0.5 kb (CCM2) from the ATG start codon