Table 1 Primers used for validation of the single nucleotide variations identified by whole-exome sequencing
From: Identification of novel genetic variations affecting osteoarthritis patients
Gene | Forward primer (5′→3′) | Reverse primer (5′→3′) |
|---|---|---|
SELP | CCTGTGTAACACAATGCG | CTACAGCTCCTACTACTG |
FIGNL1 | ATGGTAGCAATGTCAGCAG | GAAGATCGTATCCTAGTGG |
SPN | CTCGTGCTGAGCAGAGGC | CATCCTCACTGGCCACCAG |
USP36 | CTCGTGCTGAGCAGAGGC | CTCACTGGCCACCAGTGG |
TNRC6B | TCTGGCAGAGAACAGGCTC | CACCTGTTATTGTTGTCC |
HSPG2 | GACTCTGCTATGCCATGTC | CACAGGTGGTCAGCGACAC |
SUSD5 | AGGTGTCTGCTTGACAGTG | CTAGTTACTCCTGATCAGC |
ITGA8 | GGAAGGCTAATAACGATCAG | GGTTAGTAACGTGTGTTCTC |
PXN | CATCCAAGAGACTCTCCAC | CCATCTGCTGACCTCTAG |
NLRP6 | AGCAACTGGAGCTTCGTG | GTGGCGCTCGATGTCGCGC |
COL2A1 | CTTAGTCCAGAGACTGCG | GGCCTGACAGGTCAGCTG |
COL6A6 | CATCGGTGCTGCACTCAG | CTGTGGTACAGATGTTGCG |
GAS6 | GGCACGCAGCAGATGCAG | CCCAGGCTGGTAGCTGAG |