Fig. 3From: Expression analysis of the osteoarthritis genetic susceptibility locus mapping to an intron of the MCF2L gene and marked by the polymorphism rs11842874Functional analysis of SNPs within the rs11842874 linkage disequilibrium block. (a) Schematic diagram of the 7 SNPs within the LD block. The SNPs are within an 11.4 kb region located within the MCF2L gene body (intron 3 relative to transcript isoforms 002 and 201); adapted from the UCSC genome browser. Luciferase reporter analyses were performed in (b) SW872 liposarcoma and (c) U2OS osteosarcoma cells. Cells were transfected with luciferase reporter constructs and a renilla reporter vector. Luciferase activity was quantified and normalised to renilla activity and to the empty luciferase vector. Mean luciferase/renilla data is plotted, with error bars representing standard error of the mean from six technical repeats and a minimum of six biological repeats. SNPs are presented in their physical order on the x-axis. Major (risk) alleles are presented as black filled bars, minor alleles as grey filled bars. Two fragments encompassing rs118021693 were analysed; fragment A and the larger fragment B. Statistical significance represents the difference between alleles for each SNP and was assessed using the Mann–Whitney U test, and is not corrected for multiple testing. ns not-significant, *p < 0.05, **p < 0.01, ***p < 0.001Back to article page