Fig. 1From: Expression analysis of the osteoarthritis genetic susceptibility locus mapping to an intron of the MCF2L gene and marked by the polymorphism rs11842874Analysis of relative MCF2L expression and of its protein coding transcript isoforms. a MCF2L expression measured by qPCR in cartilage, synovium and fat pad from OA patient donors. MCF2L expression was plotted relative to three housekeeping genes (GAPDH, 18S and HPRT1) using the 2-ΔCt method. Each dot represents an individual sample. Lines represent the mean value of expression and the standard error of the mean. The p value was calculated using a non-parametric Kruskal-Wallis test to highlight the differences in mean expression levels between each of the three tissue types tested. b MCF2L expression measured by qPCR in OA hip and NOF. Horizontal lines represent the mean and standard error of the mean. c MCF2L expression in OA hip and NOF cartilage as measured by RNA-seq. Expression is measured as fragments per kilobase of exon per million fragments mapped (FPKM). Horizontal lines represent the mean and standard error of the mean. For b and c, statistical significance was assessed using the Mann–Whitney U test and is not corrected for multiple testing. d MCF2L protein-coding transcript isoform expression as measured by RNA-seq in the OA hip (grey bars) and NOF cartilage (black bars) samples used in c. e Publically available MCF2L protein-coding transcript isoform expression as measured by RNA-seq in two fibroblast-like synoviocyte (FLS) samples: SRX105525, shown as black filled bars, and SRX105526, shown as grey filled bars. f Publically available MCF2L protein-coding transcript isoform expression as measured by RNA-seq in synovial macrophages co-cultured with FLS. g Publically available MCF2L protein-coding transcript isoform expression as measured by RNA-seq in synovial macrophages co-cultured with FLS in the presence of tumour necrosis factor (TNF). For d-g, the isoforms are labelled with their Ensembl ID on the x-axis and are in the order listed in Additional file 3. Their relative expression, measured as FPKM, is on the y-axis. ‘MCF2L gene’ represents the aggregated expression level of all isoformsBack to article page