Fig. 2From: Polymorphism in microRNA-binding site in HNF1B influences the susceptibility of type 2 diabetes mellitus: a population based case–control studyEffect of the base substitutions due to SNPs rs2229295 and rs1800929 on miRNA binding. a Schematic representation of reporter constructs used in the luciferase reporter assay. Plasmid construct containing TC sequence, which was selected randomly, was used as a reference. Major allele (C for rs2229295) is shown in blue and minor allele (A for rs2229295) is shown in red. b Relative luciferase activity of each reporter construct. Luciferase activity was normalized to Renilla luciferase levels. Luciferase activities relative to the reference vector (TC vector) are shown as mean ± S.E. from three independent transfection experiments with triplicate assays. The luciferase activities among four constructs were compared using the Turkey-Kramer method (*p < 0.05, **p < 0.01)Back to article page