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Fig. 3 | BMC Medical Genetics

Fig. 3

From: Biochemical effects of mutations in the gene encoding the alpha subunit of eukaryotic initiation factor (eIF) 2B associated with Vanishing White Matter disease

Fig. 3

Effects of eIF2Bα mutations on GEF activity and inhibition by phosphorylated eIF2. a eIF2B GEF activity assays were carried out on complexes containing the mutant eIF2Bα subunits that do not affect eIF2B complex formation. Data are shown as the GEF activity relative to WT, which is set to 100 %. Activities are shown as mean ± SEM of six independent experiments. P-values were calculated by t-test with Welch’s correction. b Western blot showing the increase in eIF2α phosphorylation following treatment with PKR. c eIF2B GEF activity assays were carried out on complexes containing WT or Asn208Tyr eIF2Bα using either unphosphorylated or in vitro phosphorylated eIF2. Data are shown as the GEF activity relative to WT, which is set to 100 %. Activities are shown as mean ± SEM of four independent experiments. P-values were calculated by t-test with Welch’s correction. d The data from (c) with the activity for assays with unphosphorylated eIF2 set to 100 % in order to compare the relative decrease in activity

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BMC Medical Genetics

ISSN: 1471-2350

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