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Fig. 4 | BMC Medical Genetics

Fig. 4

From: The role of p19 and p21 H-Ras proteins and mutants in miRNA expression in cancer and a Costello syndrome cell model

Fig. 4

P19 H-Ras does not activate cell growth. DKO H-Ras(−/−)/N-Ras(−/−) fibroblasts that stably express pEGFP (negative control), pEGFP-p19 and pEGFP-p21 were studied by direct cell-proliferation assay. Cells were harvested and plated in 96 wells (10,000 cells/well) on six microplates in sextuplicate and incubated at 37 °C, 5 % CO2 with DMEM/10 % FCS. After the desired time, the microplates were washed and frozen. Cells were quantified with the green fluorescent dye CyQuant (Invitrogen) according to the manufacturer’s instructions. Fluorescence measurements were performed using a microplate reader with excitation at 485 nm and detection at 530 nnm

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