Figure 3

Real-Time PCR confirms differences in gene expression in colonic epithelium and stroma. Quantitative real-time RT-PCR was done on both biopsies (taken at two separate visits) from 10 study participants for four candidate genes. The histogram bars from the same individual are located next to each other, i.e., samples 1 and 2 are from the same person but taken at two separate visits. Total RNA was reverse transcribed and then amplified by PCR and normalized to β-glucuronidase (GUSB) expression. Blue bars -epithelial tissue. Red bars -stromal tissue. Upper panel shows expression of (i) ABCC3, an epithelial gene involved in drug and lipid metabolism and (ii) MLPH (melanophilin), an epithelial gene involved in protein binding and protein transport. Lower panel shows expression of (iii) ANXA1 (annexin A1), a stromal gene involved in cell migration and cell adhesion and (iv) ITGA8 (integrin α8), a stromal gene involved in extracellular matrix formation.