Figure 1

Schematic location of the rs4803217 SNP in relationship to other SNPs in and near the IFNL2/3 gene. All four variants are in high linkage disequilibrium in Caucasians (r²≥0.92) [12]. The linkage disequilibrium between the four markers is represented in the table below the diagram and displayed as r² (10;32), with a lower linkage disequilibrium reported between rs12979860 and rs8099917[11]. (A) The nucleotide sequence around rs4803217 is shown for IFNL3 and IFNL2 with primers designed to be specific for IFNL3 (B). The IFNL3 specific forward primer contains a mismatch to IFNL2 at position 11 (from the 5’end) for IFNL3 specificity. To distinguish A and C allele of the rs4803217 SNP, the A specific primer ends with A at the 3' end, and the C allele specific primer ends with C at the 3' end. The reverse common primer contains one IFNL3 specific mismatch to IFNL2 at the 3^rd base from the 3' end. Mismatches between IFNL3 and IFNL2 are highlighted by asterix*, and the IFNL3 specific base within each primer is underlined.