Figure 3
From: Functional analysis of the novel TBX5c.1333delC mutation resulting in an extended TBX5 protein
Co-localization of wild type and mutant TBX5 with SALL4. The subcellular distribution of the proteins was analyzed in COS-7 cells transfected with either wild type or mutant TBX5-GFP constructs and SALL4-FLAG. SALL4 was detected using mouse α-FLAG antiserum and a secondary goat anti-mouse TRITC-labelled antibody. SALL4 and TBX5 (A, B, C) form overlapping distinct structures in the nucleus. The SALL4 signal was also shown to interfere with subcellular distribution of the mutated TBX5 in dot-like structures in the nucleus (D, E, F). The nuclei, stained with DAPI, appear in blue.