Minisequencing mitochondrial DNA pathogenic mutations

BMC Medical Genetics

Table 2 Amplification primers

POSITIONS	FORWARD	REVERSE	SIZE (bp)	Final Concentration (μM)
3243	tatacccacacccacccaag	ggccatgggtatgttgttaag	118	0.2
3460	ccgaacgaaaaattctaggc	gcggtgatgtagagggtgat	153	0.2
3697	gcctagccgtttactcaatcc	tgagattgtttggggctactgc	94	0.15
3946/3949¹	tagcagagaccaaccgaacc	gaagattgtagtggtgagggtgt	157	0.2
7445	ccctaccacacattcgaagaa	tggcttgaaaccagctttg	89	0.2
8993	aatgccctagcccacttctt	aggtggcctgcagtaatgtt	140	0.15
9176	aaatcgctgtcgccttaatc	tcattaggagggctgagagg	154	0.3
10158/10191	tcaacaccctcctagcctta	gggtaaaaggagggcaattt	196	0.3
10663	acacccactccctcttagcc	ggccatatgtgttggagattg	110	0.3
11777/11778/11832	cacgggcttacatcctcatt	gggggtaaggcgaggttag	157	0.2
12706¹	tgtagcattgttcgttacatgg	agttggaataggttgttagcgg	146	0.2
13513/13514	attggcagcctagcattagc	cagggaggtagcgatgagag	131	0.2
14459/14482/14484/14487	ctccatcgctaaccccacta	ttctgaattttgggggaggt	170	0.4

ISSN: 1471-2350