Figure 3

Pre- and postnatal plasma and urine methylmalonic acid levels. (A) MMA concentrations (μM) measured in the plasma over time. Error bars surround the standard deviation. Wild-type (N = 4–8 age matched littermates at each time point, not displayed), Mut null prenatal [E19] (N = 3), Mut null postnatal 4–6 hours (N = 4), Mut null postnatal 8–12 hours (N = 4), and Mut null postnatal 20–24 hours (N = 4). Low-level wild-type values not displayed. An asterisk * designates a significant p-value, calculated using a one-way ANOVA with Tukey-Kramer adjustment, for comparison between advancing time points. Prenatal (value = 176 μM) vs. 4–6 hours [F (1,6) = 107; p < 0.0001], 4–6 hr vs 8–12 hours [F (1,6) = 54; p < 0.0001] and 8–12 hours vs 20–24 hours [F (1,4) = 152; p < 0.0001]. At all time points, the mutants were different from the unaffected littermates with p-values less than 0.001. (B) MMA concentrations (mM) in urine or amniotic fluid on day E19 (value = 117 μM) from the samples above. An asterisk * designates a significant p-value, calculated using a one-way ANOVA with Tukey-Kramer adjustment, for comparison between advancing time points. Prenatal vs. 4–6 hours [F (1,6) = 30; p = 0.0002] and 4–6 hr vs 8–12 hours [F (1,6) = 49; p < 0.0001]. However, at 8–12 hours vs 20–24 hours the differences were not significant [F (1,4) = 0.14; p = 0.7143]. In all cases, the mutants were different from the unaffected littermates (not displayed) with p-values less than 0.001.