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Table 3 WAVE conditions used for screening TGFBR1 and TGFBR2 genes.

From: Resequencing of genes for transforming growth factor β1 (TGFB1) type 1 and 2 receptors (TGFBR1, TGFBR2), and association analysis of variants with diabetic nephropathy

Primer Set

Tm°C

Melt Range°C

Run Temperature°C

WAVE Buffer B (%)

TGFBR1 i

59.4

54 – 72

57

69–79

   

59

67–77

   

64

62–64

TGFBR1 ii

63.3

60 – 72

63

68–78

   

69

62–72

TGFBR1 iii

69.2

58 – 75

69

68–78

   

72

65–75

TGFBR1 iv

55.9

54 – 65

55

68–78

   

59

64–74

TGFBR1 v

57.2

54 – 65

55

62–72

   

59

58–68

   

61

56–58

TGFBR1 vi

56.1

54 – 65

56

68–78

   

59

65–75

TGFBR1 vii

56.5

54 – 68

55

69–79

   

58

66–76

TGFBR1 viii

57.0

54 – 65

57

67–77

TGFBR1 ix

57.4

55 – 67

56

67–77

   

58

64–75

   

62

61–71

TGFBR1 x

54.8

51 – 66

54

65–75

   

57

62–72

TGFBR1 xi

54.6

54 – 67

54

65–75

   

58

60–70

TGFBR1 xii

56.9

55 – 65

56

64–77

   

58

62–72

TGFBR1 xiii

55.8

52 – 62

56

67–77

TGFBR1 xiv

55.2

54 – 63

55

67–77

   

59

63–73

TGFBR2 i

66.1

59–74

60

63–73

   

65

58–68

   

67

56–66

   

70

53–63

TGFBR2 ii

66.3

61 – 72

64

58–68

   

66

56–66

   

69

52–62

TGFBR2 iii

58.5

57 – 61

59

61–71

   

61

59–69

TGFBR2 iv

59.8

55 – 65

56

67–77

   

61

60–70

   

63

58–68

TGFBR2 v

60.5

57–64

62

58–68

TGFBR2 vi

63.5

62–65

64

55–65

TGFBR2 vii

59.7

53–71

59

57–67

   

61

52–62

TGFBR2 viii

59.7

53 – 71

59

66–76

   

61

64–74

TGFBR2 ix

61.0

60–61

61

57–67

TGFBR2 x

57.0

51–66

52

63–73

   

58

57–67

   

62

53–63

   

64

50–60

TGFBR2 xi

54.4

53 – 66

54

67–77

   

56

65–75

   

60

61–71

TGFBR2 xii

57.0

56–60

59

63–73