Primer Set | Tm°C | Melt Range°C | Run Temperature°C | WAVE Buffer B (%) |
---|---|---|---|---|
TGFBR1 i | 59.4 | 54 – 72 | 57 | 69–79 |
59 | 67–77 | |||
64 | 62–64 | |||
TGFBR1 ii | 63.3 | 60 – 72 | 63 | 68–78 |
69 | 62–72 | |||
TGFBR1 iii | 69.2 | 58 – 75 | 69 | 68–78 |
72 | 65–75 | |||
TGFBR1 iv | 55.9 | 54 – 65 | 55 | 68–78 |
59 | 64–74 | |||
TGFBR1 v | 57.2 | 54 – 65 | 55 | 62–72 |
59 | 58–68 | |||
61 | 56–58 | |||
TGFBR1 vi | 56.1 | 54 – 65 | 56 | 68–78 |
59 | 65–75 | |||
TGFBR1 vii | 56.5 | 54 – 68 | 55 | 69–79 |
58 | 66–76 | |||
TGFBR1 viii | 57.0 | 54 – 65 | 57 | 67–77 |
TGFBR1 ix | 57.4 | 55 – 67 | 56 | 67–77 |
58 | 64–75 | |||
62 | 61–71 | |||
TGFBR1 x | 54.8 | 51 – 66 | 54 | 65–75 |
57 | 62–72 | |||
TGFBR1 xi | 54.6 | 54 – 67 | 54 | 65–75 |
58 | 60–70 | |||
TGFBR1 xii | 56.9 | 55 – 65 | 56 | 64–77 |
58 | 62–72 | |||
TGFBR1 xiii | 55.8 | 52 – 62 | 56 | 67–77 |
TGFBR1 xiv | 55.2 | 54 – 63 | 55 | 67–77 |
59 | 63–73 | |||
TGFBR2 i | 66.1 | 59–74 | 60 | 63–73 |
65 | 58–68 | |||
67 | 56–66 | |||
70 | 53–63 | |||
TGFBR2 ii | 66.3 | 61 – 72 | 64 | 58–68 |
66 | 56–66 | |||
69 | 52–62 | |||
TGFBR2 iii | 58.5 | 57 – 61 | 59 | 61–71 |
61 | 59–69 | |||
TGFBR2 iv | 59.8 | 55 – 65 | 56 | 67–77 |
61 | 60–70 | |||
63 | 58–68 | |||
TGFBR2 v | 60.5 | 57–64 | 62 | 58–68 |
TGFBR2 vi | 63.5 | 62–65 | 64 | 55–65 |
TGFBR2 vii | 59.7 | 53–71 | 59 | 57–67 |
61 | 52–62 | |||
TGFBR2 viii | 59.7 | 53 – 71 | 59 | 66–76 |
61 | 64–74 | |||
TGFBR2 ix | 61.0 | 60–61 | 61 | 57–67 |
TGFBR2 x | 57.0 | 51–66 | 52 | 63–73 |
58 | 57–67 | |||
62 | 53–63 | |||
64 | 50–60 | |||
TGFBR2 xi | 54.4 | 53 – 66 | 54 | 67–77 |
56 | 65–75 | |||
60 | 61–71 | |||
TGFBR2 xii | 57.0 | 56–60 | 59 | 63–73 |