Table 1 PCR and sequencing primers for human PNMT gene
Primers | Sequence 5'-3' |
|---|---|
PCR primers for the first and second gene fragments | |
PNMT_PCR1_F | AACCCGAACCTTCTGTCCTC |
PNMT_PCR1_R | CAGAGTTAGACTGAACCCAGCTC |
PNMT_PCR2_F | GCTCAGAATTGAGAGCTAAGGTG |
PNMT_PCR2_R | TGTTTGTGACTTCACCTCTCTGA |
Sequencing primers for the first PCR fragment | |
PNMT_seq1_F | CTAAGTGCATTAGCACAGCTCAC |
PNMT_seq1_R | ATCCTCCCCACCCATTCATC |
PNMT_seq2_F | GTCTAAAGATTGTGGGGGTGAG |
PNMT_seq2_R | CTCTCCTAAGGGATGTTGCTCTT |
PNMT_seq3_F | ACGAGGGACAAGAGGTCGT |
PNMT_seq3_R | GTGGATCCTAAGGTTGGGAGTT |
Sequencing primers for the second PCR fragment | |
PNMT_seq4_F | ATAGGAGGAAATGGAGGCAGA |
PNMT_seq4_R | CCTGAACCAATGTCGATGAG |
PNMT_seq5_F | TTGCAGAGGAGAAGGAAGAACTA |
PNMT_seq5_R | TCAGCAGCGTGGTGATGT |
PNMT_seq6_F | TGCTGGCAGGATAAGGAG |
PNMT_seq6_R | AAAAAGCCTAGGGTGAATGTCTC |