From: Functional analysis of splicing mutations in exon 7 of NF1gene
Mutation | Mutagenic primer plus | Mutagenic primer minus |
---|---|---|
R304X | 5'-AAGTTATTTCTGGACAGTCTATGAAAAGCTCTTGCTGGCCATGG-3' | 5'-CCATGGCCAGCAAGAGCTTTTCATAGACTGTCCAGAAATAACTT-3' |
Q315Q | 5'-GGCCATGGAGGAAGTAGGCAACTGACAGAAAGTGCTGCAATTG-3' | 5'-CAATTGCAGCACTTTCTGTCAGTTGCCTACTTCCTCCATGGCC-3' |
L316M | 5'-GGCCATGGAGGAAGTAGGCAGATGACAGAAAGTGCTGCAATTG-3' | 5'-CAATTGCAGCACTTTCTGTCATCTGCCTACTTCCTCCATGGCC-3' |
Q315Q+L316M | 5'-GGCCATGGAGGAAGTAGGCAAATGACAGAAAGTGCTGCAATTG-3' | 5'-CAATTGCAGCACTTTCTGTCATTTGCCTACTTCCTCCATGGCC-3' |
N335N | 5'-GTAAAGCAAGTACTTACATCAACTGGGAAGATAACTCTGTC-3' | 5'-GACAGAGTTATCTTCCCAGTTGATGTAAGTACTTGCTTTAC-3' |