Figure 3
From: Functional analysis of splicing mutations in exon 7 of NF1gene
a. Post transfection PCR products of the minigene constructs obtained by SD6 and SA2 primers. The upper band indicates a 435 bp product encompassing exon 7. The lower band indicates a 261 bp product with exon 7 skipping. The central band corresponds to an alternative product of 403 bp. b. Quantification by Quantity One software of the bands showed on the top. The intensity of each band, representing the amount of each transcript, is expressed as percentage.