Table 1 Primers used in the analysis of the EDAR gene. Intronic primer sequences, annealing temperatures and sizes of PCR products suitable for DHPLC analysis and DNA sequencing of the EDAR gene.
From: EDAR mutation in autosomal dominant hypohidrotic ectodermal dysplasia in two Swedish families
Exon no. | Forward primer (5'-3') | Reverse primer (5'-3') | Optimal annealing temp.* (°C) | Fragment size (bp) |
|---|---|---|---|---|
2 | TTTGCTGGAAGGCACCTTAT | AGAGGCCAAGAAACAGTCCA | 58–62 | 243 |
3 | ACCCCCTTCCTATGTCAACC | CAGGCTCAGGGCAACAAT | 56–62 | 292 |
4 | CGGCAAGAGTAGCTTCTGGA | GCAGTATCCATGACCCCTGT | 51–63 | 397 |
5 | GTGCTCTCTGCACCAGTCC | GACCGGCTCTTTCCTACACC | 52–63 | 246 |
6 | AGCTCTGTGGCAGCGTCT | CCTCTCCTCTTCTGAGCTTTCA | 51–62 | 228 |
7 + 8 | GGAGTCCTGGAGGGAAGACC | AGCATGTGAGAGCAGAAGCA | 60 | 468 |
9 | AGAGCAGGGTTGGGCTGAG | GCTAGCCTGTCAGTTCACTCG | 51–63 | 248 |
10 | AGGTGCCCAGTAAACACCTG | CGTCTTGCAGGAGAGCTGAT | 51–63 | 400 |
11 | CCTGCTGACATGGAGGATTT | CTCAGTTCCCCTCACAGGAG | 51–63 | 234 |
12 | GACCTTCTATTGACTGTGACTTGC | CAGTCTTTTGGCACCACTCA | 51–63 | 461 |