From: Three allele combinations associated with Multiple Sclerosis
Gene/marker | Chromosome localization | Polymorphism type* [refSNP ID]** | Names of alleles considered | Method of analysis (the restriction endonuclease used) | Numbers of genotyped MS patients/controls |
---|---|---|---|---|---|
DRB1 | 6p21 | Allele groups corresponding to serological specificities DR1-DR18(3) | 01–18(3) [44] | PCR-SSP | 229/314 |
Microsatellite TNFa | 6p21 | (AC)n | a1-a13[23] | Nested PCR | 121/103 |
Microsatellite TNFb | 6p21 | (TC)n | b1-b7[23] | Nested PCR | 120/96 |
 |  | SNP -376A→G [rs1800750] | A, G | PCR-SSP | 202/146 |
TNF | 6p21 | SNP -308G→A [rs1800629] | A1, A2 | PCR-SSP | 223/222 |
 |  | SNP -238A→G [rs361525] | B1, B2 | PCR-RFLP (BamHI) | 165/112 |
LT | 6p21 | SNP +252G→A | N1, N2 | PCR-RFLP (NcoI) | 205/150 |
 |  | SNP +319C→G | H1, H2 | PCR-RFLP (AspHI) | 202/147 |
 |  | SNP -509C→T [rs17551290] | C, T | PCR-SSO | 119/295 |
 |  | SNP +72 wild type→C insertion | wt, ins |  | 198/340 |
TGFβ1 | 19q13 | SNP +869T→C (10Leu→Pro) | T, C |  | 150/248 |
 |  | SNP +915G→C (25Arg→Pro) | G, C |  | 157/248 |
 |  | SNP +1632C→T (263Thr→Ile) | C, T |  | 178/109 |
CCR5 | 3p21 | Wild type→32 base pair deletion | wt, Δ 32 | PCR | 221/355 |
CTLA4 | 2q33 | SNP +49A→G (17Thr→Ala) [rs231775] | A, G | PCR-RFLP (BstEII) | 168/209 |