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Table 3 Conditions and the Primers Sequences

From: Analysis of coding variants in the betacellulin gene in type 2 diabetes and insulin secretion in African American subjects

SNP

FORWARD PRIMER 5' to 3'

REVERSE PRIMER 5' to 3'

SEQUENCE PRIMER 5' to 3'

Anneal Temp.

C7G

*CGAAGAAGGAGGGAGACTT

AATGACTTTCCTCCTGGTTTCCA

GAGCTGGCGCCGCTGC

55°C

L44F

TAGACTGTTTCACAATAAGC

*ACAGTGACTGGTACCTTA

GAAGTCCTGAAACTAATGGC

50°C

L124M

CATTGGAGCAAGGTGTGAGA

*CATGTGCAGACACCGATGA

ACAGATTCTGGTGATTTGT

55°C

  1. * Denotes addition of M13 primer sequence (5'-CACGACGTTGTAAAACGAC-3') to the 5' of primer.
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BMC Medical Genetics

ISSN: 1471-2350

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