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Table 1 Oligonucleotide primers used for PCR amplification and DNA sequencing of the exons of the ITGA8, FRMD4A, and LAMR1P6 genes.

From: Arrhythmogenic right ventricular cardiomyopathy type 6 (ARVC6): support for the locus assignment, narrowing of the critical region and mutation screening of three candidate genes

Gene Exon No. PCR fragment size (bp) Forward primer (5' → 3') Reverse primer (5' → 3') Melting temperature (°C)
ITGA8 1 587 acc gcc aga ttc cac cag tgggtc ttc tgg gtc ctg t 58
  2 300 ggg cag gag gtt aca gag ac acc aag aca gcg gga agt c 56
  3 300 gag gag ttt gac aag gat tgg gct ttc cta aac atg agc ttg 54
  4 368 ggc caa tta att cac cca at tac cta ccc ccg aag ctt tt 48
  5 399 tca caa cgc tat ttg gtg taa ga cag gct agg tat gtt cct caa ga 58
  6 223 tct ctc aag gga gag agt tca a tgt ttg agg tga tgg tta tgc t 45
  7 296 ttt caa ggg aat gtg ggt ct cat cgg ata agt cag gga ta 54
  8 249 tgg tag ccc ttt cac ctt tt ctc cat taa tat ccc ttt gg 56
  9 250 ata tgg cgt gtg tgt gtg tg cgg tgg att agg tgt att aag tg 54
  10 238 cca att cta tgg caa atg gt tgc cta gga cag ttt cca gt 56
  11 329 att cac tgg gtc agg ggt tag tga ggc aat aag gaa ggg tgt 58
  12 464 taa caa agc agc gaa agc aa taa ctg cac cca cac aca cc 58
  13 475 tag aga agt tga ggc agg agg atc act tg gct tca gcc tca tct gta gaa gaa aac tgc 56
  14 250 acc atc agc atg tag ggg ta ccc cag aat aaa tcg ttg ga 55
  15 243 Atg cag tga agt ggg ctt ct caa gat acc cag gtg atg tga a 55
  16 553 ttg gta gaa ggg gtg tgt g gca tga taa atg agc tgg aa 56
  17 468 ttc cag ctc att tat cat gc gaa gtc agg gtc aag agg ag 55
  18 399 aca taa atc agc ctc cat cta agc cat gca gaa gta aaa atg aaa gga 56
  19 278 ttt gtg gca cca ttc aga att t caa gaa ttt cac gca gca tag tt 56
  20 460 gat gat tct ctt gcc tct tcc tac gtg gct cat tgc tgt tat gat tta 56
  21 299 ctc ctg ggt gtt tga gat cc agg tgc tga gtt gct gag tg 58
  22 244 ggg ttt tgc agg aat gac tt aag ggc ctt gac aga tga c 58
  23 268 cgt gtg ttc tcc tgt gat atg cat cag gaa aat tcc aca tca 55
  24 324 gaa aac cac ttt ctt ctg ctt c cac agc gag atc ctg tct ca 56
  25 396 tgc cat atc gac ttt gaa tt aat caa caa agc cac tga 55
  26 369 cac agg tct gga tcc tca tgt tga gca ctg aag gag acc aa 58
  27 298 ggt gat gac ttt tga gga aat ga cgc tat gaa aag act tcc cac t 58
  28 263 att agg tgg ctg cca gta taa c acc act aaa gcc tag cac aag c 56
  29 380 gga tcc ttt cag atc aac ttc c gca aac aag aca cga ttt tcc 56
  30 365 ctg ggg aat aga gag cga gac gtg cgg tgt aga tga ggt gat 58
FRMD4A 1 597 acc tcc tat gcc tcc ctg aa ctg gca gca att ctt cct tt 58
  2 371 cac agt tcc cac cct gtc tt aaa gag cct gcg gga taa ag 58
  3 395 ata cag tgc cga ctc cca ga gga tgc ctt agg ctg gtg ta 58
  4 387 gac cct tga ctt ggc tgt gt caa gtg gtc tgc agc tga tt 58
FRMD4A splice variant 1 688 ggt gag cat gtg acc tac tgt cca act gtt cag gaa acg tg 58
  nested primers   gc gtg act tgc atg atg tga c ccc aag cct tat agc act aaa c  
LAMR1P6 1 884 ttc cca tcg caa ctt aaa gg caa cgt tgt ttc cat gtt gc 58
  nested primers   aca gag caa tgg tgg gaa ag ctt tcc cac cat tgc tct gt  
  nested primers   aat tgc tgg cca ctt cac tc caa ctg cat caa acc cac tg