Table 5 Analysis of SNPs in LMNA. Details of primers and probes used in TaqMan® analysis and PCR conditions for direct sequencing.
From: Aging syndrome genes and premature coronary artery disease
SNPs typed by TaqMan® | |||||
|---|---|---|---|---|---|
SNP ID | Position | Forward Primer (5'-3') | Reverse Primer (5'-3') | TaqMan ® probe (VIC) | TaqMan ® probe (FAM) |
rs955383 | 2,533 | GGCCAGGAGTTTTAGACCAAATT | TGAGTAGCTGAGACTATAGGCTATCATG | ATAGCAAGgCTCCGTC | TAGCAAGaCTCCGTCTC |
rs2485662 | 3,971 | ACTACCTTCTTTCTGGCTGAAACCAG | GGGGAAGCAGGGCTGGG | AGACCCAAtATTGGCT | ACCCAAcATTGGCT |
rs517606 | 9,827 | CGAACTCCTAGGCTCAAGTAATCC | GGGCTACAGACTAGAAAGAGACAGAGA | CTGGGATgTATAGGCA | CTGGGATtTATAGGCATGA |
rs593987 | 16,890 | TTGCTGTGCTGGTGCCTTT | GAGTTGGCACTTGCAAATGTGA | AGCCgGACTTCCT | AGCCaGACTTCCTTG |
rs528636 | 19,123 | TCTAAATTCTGAGAGCCTCCTAGTACA | GCAACTTAGATTCCGAGCTCCTT | AGCAGCCaTTAGC | AGCAGCCgTTAGC' |
rs508641 | 20,642 | GGAGAGAGAGGGAAAAGCATTCA | TGACTCAGGGCTCAGGAACGT | ACGGGGtAGAGCT | ACGGGGcAGAGCT |
rs553016 | 27,366 | GCTTGGGACTCTGGGGAG | CTTCCACACCAGGTCGGTA | TCCCATCgCCACCCA | TCCCATCaCCACCCA |
rs4641 | 28,037 | CGAGGATGAGGATGGAGATGA | TCAGCGGCGGCTACCA | TCCATCACCACCAcGTG | CCATCACCACCAtGTG |
rs7339 | 29,479 | CAGAACTGCAGCATCATGTAATCTG | GGGTTATTTTTCTTTGGCTTCAAG | CCTGCACgTCATGG | CCTGCACcTCATGG |
rs3204564 | 30,139 | AGGTGGAAGAAGGGAGAAGAAAG | GCACCCCACTTGGCTTCA | CCTAGCTTTAgACCCTGG | CCTAGCTTTAaACCCTGG |
rs536857 | 31,790 | CACTGTGGGCTGGGGAACAC | CCTCAGCCCTCCTCCTCAAGAG | AGCAGGCaACGTT | AGCAGGCgACGTT |
SNPs typed by direct sequencing | |||||
SNP ID | Position | Primers(5'-3') | PCR conditions | ||
rs568036 | 30,669 | These SNPs are contained in the amplicon amplified by the primers 5'-ACTGCATCCTCCTGCTCATT-3' and 5'-GGCTCCTACTTGGCCTAACA-3' | 95°C for 2 min, followed by 35 cycles of 94°C for 30 s,60°C for 30 s, and 72°C for 90 s, followed by a 20 min 72°C final extension. | ||
rs568035 | 30,670 | ||||
rs6669212 | 30,816 | ||||